UC
GENÉTICA MOLECULAR Y MICROBIOLOGÍA

LARRONDO CASTRO,LUIS FERNANDO

Luis Fernando Larrondo Castro Luis Fernando Larrondo Castro

Laboratorio: 3541916
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Profesor Asociado

GENÉTICA MOLECULAR Y MICROBIOLOGÍA,
Facultad de Ciencias Biológicas,
Alameda 340, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.

Antecedentes Académicos

Doctor (Biologia celular y molecular) , Pontificia Universidad Católica de Chile (2003).
Magister (Master in Biochemistry) , Pontificia Universidad Católica de Chile (1997).
Licenciado (Biochemistry) , Pontificia Universidad Católica de Chile (1995).

Postdoctorado:

Departamento de Genética, Dartmouth Medical School. Hanover, N.H. EE.UU.

Especialización:

Ritmos circadianos y fotobiología. Genética y Genómica Funcional de Hongos

Premios y Distinciones

  • Postdoctoral Latin American Fellowship in the Biomedical Science, Pew Latin American Fellows Program ().
  • Fellowship from the Genetics Society of America (GSA), Genetics Society of America (GSA) ().
  • Medalla Herman Niemeyer, Sociedad Chilena de Biología (2002).
  • Fellowship from the Genetics Society of America (GSA), Genetics Society of America (GSA) ().
  • Travel fellowship, Department of research and Postgraduate Research (DIPUC) at the Pontificia Universidad Cat6Jica de Chile ().
  • Graduate Fellowship, Fundación Andes ().

Actividades de Docencia

Pregado
  • 2017 BIO288C-1, Genetica Molecular.
  • 2016 BIO288C-1, Genetica Molecular.
  • 2015 BIO288C-1, Genetica Molecular.
Postgrado
  • 2017 BIO4026-1, Biología Sintética.
  • 2016 BIO4026-1, Biología Sintética.
  • 2015 BIO4026-1, Biología Sintética.

Líneas de Investigación

Nuestro laboratorio está interesado en el estudio de la regulación circadiana de la expresión génica. Mediante técnicas de genética clásica, biología celular y high-throughput screenings basados en sistemas reporteros de luciferasa, estamos explorando los mecanismos involucrados en regulación de los miles de genes controlados por el oscilador central.

Los ritmos circadianos son ritmos biológicos con un periodo cercano a las 24 horas, contenidos a nivel celular, con mecanismos de compensación de temperatura y que son reguladores biológicos clave en prácticamente todos los organismos vivientes. La disrupción de la regulación circadiana tiene consecuencias fisiológicas que han sido correlacionadas con diversas condiciones, incluyendo depresión, cáncer o la conocida experiencia de jetlag, experimentada por viajeros frecuentes.

Aunque los relojes circadianos han emergido al menos en tres ocasiones independientes durante la evolución, en todos los casos se han centrado en el mismo tipo de diseño: la presencia de un feedback loop transcripcional-traduccional negativo, confeedback loops positivos interconectados. Así, los hallazgos en un organismo son fácilmente extrapolables a otro modelo experimental, facilitando el entendimiento de la compleja maquinaria molecular detrás de la propiedad emergente de la medición del tiempo a nivel celular y sistémico.

Algunas de las preguntas que nuestra investigación intenta contestar incluyen: ¿Cuáles son los mecanismos transcripcionales que llevan a la expresión rítmica de genes en forma diaria? ¿Qué determina que un gen sea expresado en la noche y no en el día? Como viaja la información temporal desde el oscilador circadiano hacia los genes blanco dispersos a través del genoma? Para abordar estas interrogantes utilizamos como modelo de estudio el ascomicete Neurospora crassa, un organismo que ha jugado un rol clave en el estudio de la biología circadiana. Este hongo es un excelente organismo modelo: su genoma ha sido totalmente secuenciado, se han generado knockouts para cerca del 80% de sus casi 10,000 genes y es posible utilizar técnicas de genética directa y reversa. Esto sumado a su condición haploide, la diversidad de técnicas moleculares disponible así como la facilidad para su manipulación genética, facilitan tremendamente el análisis de sistemas complejos, como lo son los relojes biológicos.

En general, nuestro objetivo es entender los mecanismos moleculares que están involucrados en la interacción de un organismo con el medio ambiente. Así también, nos interesa comprender como la información ambiental permite la modificación y sincronización de los mecanismos intracelulares que conforman el reloj circadiano y como éste, a su vez, es capaz de impactar diversos programas transcripcionales, regulando así la habilidad del organismo de interactuar con su entorno.

Por otra parte nuestro laboratorio está comprometido en la utilización de los recursos generados por los distintos proyectos genoma de hongos y, de hecho, hemos estado involucrados en la anotación de diversos genomas tanto de asco como basidiomicetes. Mediante análisis genómicos y estudios comparativos deseamos poder encontrar los puntos de convergencia y divergencia de la regulación circadiana en hongos.


Titulo: Yeast Platforms For The Production Of Natural Flavor
Año: 2013
Concurso e institución: Regular, CONICYT
Investigador principal: Agosin Trumper Eduardo
Otros investigadores: Agosin Trumper Eduardo, Larrondo Castro Luis Fernando
Fecha inicio: 01-03-2013
Fecha termino: 01-03-2017
Duración: 48 meses

Titulo: Environmental Signals And Gene Expression In Neuropora Crassa: Rational Approach For The Mapping Of Transcriptional Networks And Rewiring Of Key Components In Order To Assess Basic Circadian Principles
Año: 2013
Concurso e institución: Regular, CONICYT
Investigador principal: Larrondo Castro Luis Fernando
Otros investigadores: Larrondo Castro Luis Fernando
Fecha inicio: 01-03-2013
Fecha termino: 01-03-2017
Duración: 48 meses

Titulo: Modulation Of Botrytis Cinerea Virulence Factors: Evaluationg The Effect Of Light And Circadian-Regulation
Año: 2010
Concurso e institución: Postdoctorado, UC
Investigador principal: Canessa A Paulo
Otros investigadores: Canessa A Paulo, Larrondo Castro Luis Fernando
Fecha inicio: 01-03-2010
Fecha termino: 01-03-2011
Duración: 12 meses

Titulo: Comparative And In Vivo Studies Of The Circadian Properties Of The Phytopathogen Botrytis
Año: 2010
Concurso e institución: , Otro
Investigador principal: Larrondo Castro Luis Fernando
Otros investigadores: Larrondo Castro Luis Fernando
Fecha inicio: 01-01-2010
Fecha termino: 01-12-2010
Duración: 11 meses

Titulo: Interplay Between Circadian And Copper-Controlled Gene Expression In Neurospora Crassa
Año: 2009
Concurso e institución: , Otro
Investigador principal: Larrondo Castro Luis Fernando
Otros investigadores: Larrondo Castro Luis Fernando
Fecha inicio: 01-06-2009
Fecha termino: 01-12-2010
Duración: 18 meses

Titulo: A Neurospora Crassa Systemas-Approach: Identification Of Molecular Components Involved In Circadian-Controlled Gene Expression Through Forward And Reverse Genetics Strategies
Año: 2009
Concurso e institución: Regular, CONICYT
Investigador principal: Larrondo Castro Luis Fernando
Otros investigadores: Larrondo Castro Luis Fernando
Fecha inicio: 01-03-2009
Fecha termino: 01-03-2013
Duración: 48 meses

Titulo: Búsqueda De Proteínas Que Regulan La Expresión Del Sistema Ligninolítico Del Basidiomicete "ceriporiopsis Subvermispora" En Respuesta A Metales Y Compuestos Aromáticos
Año: 2003
Concurso e institución: Regular, CONICYT
Investigador principal: Vicuna Errazuriz Jose Rafael
Otros investigadores: Vicuna Errazuriz Jose Rafael, Lobos Camus Sergio, Larrondo Castro Luis Fernando
Fecha inicio: 01-03-2003
Fecha termino: 01-03-2007
Duración: 48 meses


Publicaciones ISI

1
Recent Advances In The Study Of The Plant Pathogenic Fungus Botrytis Cinerea And Its Interaction With The Environment
Autor(es): Castillo L, Plaza V, Larrondo L, Canessa P
Año: 2017.18:976-989.
Ref: Castillo L, Plaza V, Larrondo L, Canessa P. Recent Advances in the Study of the Plant Pathogenic Fungus Botrytis cinerea and its Interaction with the Environment. Current Protein & Peptide Science. 2017;18(10):976-989.

2
Optogenetic Switches For Light-Controlled Gene Expression In Yeast
Autor(es): Salinas F, Rojas V, Delgado V , Agosin E, Larrondo L
Año: 2017.101:2629-2640.
Ref: Salinas F, Rojas V, Delgado V , Agosin E, Larrondo L. Optogenetic switches for light-controlled gene expression in yeast. Applied Microbiology And Biotechnology. 2017;101(7):2629-2640.

3
In The Driver's Seat: The Case For Transcriptional Regulation And Coupling As Relevant Determinants Of The Circadian Transcriptome And Proteome In Eukaryotes
Autor(es): Alejandro Montenegro-Montero, Luis F. Larrondo
Año: 2016.31:37-47.10.1177/0748730415607321
Ref: Alejandro Montenegro-Montero, Luis F. Larrondo. In the Driver's Seat: The Case for Transcriptional Regulation and Coupling as Relevant Determinants of the Circadian Transcriptome and Proteome in Eukaryotes. Journal Of Biological Rhythms. 2016;31(1):37-47.

4
Circadian Clocks And The Regulation Of Virulence In Fungi: Getting Up To Speed
Autor(es): Hevia M, Canessa P, Larrondo L
Año: 2016.57:147-155.
Ref: Hevia M, Canessa P, Larrondo L. Circadian clocks and the regulation of virulence in fungi: Getting up to speed. Seminars In Cell & Developmental Biology. 2016;57:147-155.

5
Daily Magnesium Fluxes Regulate Cellular Timekeeping And Energy Balance
Autor(es): Kevin A. Feeney, Louise L. Hansen, Marrit Putker, Consuelo Olivares-Yanez, Jason Day, Lorna J. Eades, Luis F. Larrondo, Nathaniel P. Hoyle, John S. O'neill, Gerben Van Ooijen
Año: 2016.532:375-379.doi: 10.1038/nature17407
Ref: Kevin A. Feeney, Louise L. Hansen, Marrit Putker, Consuelo Olivares-Yañez, Jason Day, Lorna J. Eades, Luis F. Larrondo, Nathaniel P. Hoyle, John S. O'Neill, Gerben van Ooijen. Daily magnesium fluxes regulate cellular timekeeping and energy balance. Nature. 2016;532(7599):375-379.

6
Rim15 Antagonistic Pleiotropy Is Responsible For Differences In Fermentation And Stress Response Kinetics In Budding Yeast
Autor(es): Kessi E, Araos S, Garcia V, Salinas F, Abarca V, Larrondo L, Martinez C, Cubillos F
Año: 2016.16:1-12.doi: 10.1093/fe
Ref: Kessi E, Araos S, Garcia V, Salinas F, Abarca V, Larrondo L, Martinez C, Cubillos F. RIM15 antagonistic pleiotropy is responsible for differences in fermentation and stress response kinetics in budding yeast. Fems Yeast Research. 2016;16(3):1-12.

7
Natural Variation In Non-Coding Regions Underlying Phenotypic Diversity In Budding Yeast
Autor(es): Francisco Salinas, Carl G. De Boer, Valentina Abarca, Veronica Garcia, Mara Cuevas, Sebastian Araos, Luis F. Larrondo, Claudio Martinez, Francisco A. Cubillos
Año: 2016.22:21849-21849.doi: 10.1038/srep21849
Ref: Francisco Salinas, Carl G. de Boer, Valentina Abarca, Veronica Garcia, Mara Cuevas, Sebastian Araos, Luis F. Larrondo, Claudio Martinez, Francisco A. Cubillos. Natural Variation in Non-coding Regions Underlying Phenotypic Diversity in Budding Yeast. Scientific Reports. 2016;22(6):21849-21849.

8
Modulation Of Circadian Gene Expression And Metabolic Compensation By The Rco-1 Corepressor Of Neurospora Crassa
Autor(es): Olivares C, Emerson J, Kettenbach A, Loros J, Dunlap J, Larrondo L
Año: 2016.204:163-+.
Ref: Olivares C, Emerson J, Kettenbach A, Loros J, Dunlap J, Larrondo L. Modulation of Circadian Gene Expression and Metabolic Compensation by the RCO-1 Corepressor of Neurospora crassa. Genetics. 2016;204(1):163-+.

9
Bcpmr1 Encodes A P-Type Ca2+/mn2+-Atpase Mediating Cell-Wall Integrity And Virulence In The Phytopathogen Botrytis Cinerea
Autor(es): Canessa P , Larrondo L, Plaza V , Lagues Y, Carvajal M, Perez L, Mora H, Castillo L
Año: 2015.76:36-46.
Ref: Canessa P , Larrondo L, Plaza V , Lagues Y, Carvajal M, Perez L, Mora H, Castillo L. bcpmr1 encodes a P-type Ca2+/Mn2+-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea. Fungal Genetics And Biology. 2015;76:36-46.

10
Decoupling Circadian Clock Protein Turnover From Circadian Period Determination
Autor(es): Larrondo, L.f., Olivares-Yanez, C., Baker, C.l., Loros, J.j, Dunlap, J.c
Año: 2015.347:518-1257277.10.1126/science.1257277
Ref: Larrondo, L.F., Olivares-Yañez, C., Baker, C.L., Loros, J.J, Dunlap, J.C. Decoupling circadian clock protein turnover from circadian period determination. Science. 2015;347(6221):518-1257277.

11
The Bzip Transcription Factor Hac-1 Is Involved In The Unfolded Protein Response And Is Necessary For Growth On Cellulose In Neurospora Crassa
Autor(es): Montenegro A, Goity A, Larrondo L
Año: 2015.10:1-20.
Ref: Montenegro A, Goity A, Larrondo L. The bZIP Transcription Factor HAC-1 Is Involved in the Unfolded Protein Response and Is Necessary for Growth on Cellulose in Neurospora crassa. Plos One. 2015;10(7):1-20.

12
Around The Fungal Clock: Recent Advances In The Molecular Study Of Circadian Clocks In Neurospora And Other Fungi
Autor(es): Montenegro A, Canessa P, Larrondo L
Año: 2015.92:107-184.
Ref: Montenegro A, Canessa P, Larrondo L. Around the Fungal Clock: Recent Advances in the Molecular Study of Circadian Clocks in Neurospora and Other Fungi. Advances In Genetics, Vol 92. 2015;92:107-184.

13
A Circadian Oscillator In The Fungus Botrytis Cinerea Regulates Virulence When Infecting Arabidopsis Thaliana
Autor(es): Hevia M, Canessa P, Muller H, Larrondo L
Año: 2015.112:8744-8749.
Ref: Hevia M, Canessa P, Muller H, Larrondo L. A circadian oscillator in the fungus Botrytis cinerea regulates virulence when infecting Arabidopsis thaliana. Proceedings Of The National Academy Of Sciences Of The United States Of Ame. 2015;112(28):8744-8749.

14
Circadian Rhythms Synchronize Mitosis In Neurospora Crassa
Autor(es): Hong C, Zamborszky J, Baek M, Labiscsak L, Ju K, Lee H, Larrondo L, Goity A, Chong H, Belden W, Csikasz-Nagy A
Año: 2014.111:1397-1402.
Ref: Hong C, Zámborszky J, Baek M, Labiscsak L, Ju K, Lee H, Larrondo L, Goity A, Chong H, Belden W, Csikász-Nagy A. Circadian rhythms synchronize mitosis in Neurospora crassa. Proceedings Of The National Academy Of Sciences Of The United States Of Ame. 2014;111(4):1397-1402.

15
Bright To Dim Oscillatory Response Of The Neurospora Circadian Oscillator
Autor(es): Larrondo L, Gooch V, Johnson A, Loros J, Dunlap J
Año: 2014.29:49-59.
Ref: Larrondo L, Gooch V, Johnson A, Loros J, Dunlap J. Bright to dim oscillatory response of the neurospora circadian oscillator. Journal Of Biological Rhythms. 2014;29(1):49-59.

16
Determination And Inference Of Eukaryotic Transcription Factor Sequence Specificity
Autor(es): Weirauch M, Yang A, Albu M, Cote A, Montenegro-Montero A, Drewe P, Najafabadi H, Lambert S, Mann I, Cook K, Goity A, Larrondo L, Et Al.
Año: 2014.158:1431-1443.
Ref: Weirauch M, Yang A, Albu M, Cote A, Montenegro-Montero A, Drewe P, Najafabadi H, Lambert S, Mann I, Cook K, Goity A, Larrondo L, ET AL.. Determination and Inference of Eukaryotic Transcription Factor Sequence Specificity. Cell. 2014;158(6):1431-1443.

17
A Kinetic Study Of The Effects Of Light On Circadian Rhythmicity Of The Frq Promoter Of Neurospora Crassa
Autor(es): Larrondo L, Gooch V, Johnson A, Bourne B, Nix B, Maas J, Fox J, Loros J, Dunlap J
Año: 2014.29:38-48.
Ref: Larrondo L, Gooch V, Johnson A, Bourne B, Nix B, Maas J, Fox J, Loros J, Dunlap J. A kinetic study of the effects of light on circadian rhythmicity of the frq promoter of neurospora crassa. Journal Of Biological Rhythms. 2014;29(1):38-48.

18
Analysis Of The Phlebiopsis Gigantea Genome, Transcriptome And Secretome Provides Insight Into Its Pioneer Colonization Strategies Of Wood
Autor(es): Canessa P, Larrondo L, Hori C, Ishida T, Igarashi K, Samejima M, Suzuki H, Master E, Et Al.
Año: 2014.10:1-20.
Ref: Canessa P, Larrondo L, Hori C, Ishida T, Igarashi K, Samejima M, Suzuki H, Master E, ET AL.. Analysis of the Phlebiopsis gigantea Genome, Transcriptome and Secretome Provides Insight into Its Pioneer Colonization Strategies of Wood. Plos Genetics. 2014;10(12):1-20.

19
Conserved Rna Helicase Frh Acts Nonenzymatically To Support The Intrinsically Disordered Neurospora Clock Protein Frq
Autor(es): Larrondo L, Hurley J, Loros J, Dunlap J
Año: 2013.52:832-843.
Ref: Larrondo L, Hurley J, Loros J, Dunlap J. Conserved RNA helicase FRH acts nonenzymatically to support the intrinsically disordered neurospora clock protein FRQ. Molecular Cell. 2013;52(6):832-843.

20
Assessing The Effects Of Light On Differentiation And Virulence Of The Plant Pathogen Botrytis Cinerea: Characterization Of The White Collar Complex
Autor(es): Canessa P, Hevia M, Larrondo L, Schumacher J, Tudzynski P
Año: 2013.12:1-17.
Ref: Canessa P, Hevia M, Larrondo L, Schumacher J, Tudzynski P. Assessing the Effects of Light on Differentiation and Virulence of the Plant Pathogen Botrytis cinerea: Characterization of the White Collar Complex. Plos One. 2013;12(8):1-17.

21
Environmental Responses And The Control Of Iron Homeostasis In Fungal Systems
Autor(es): Canessa P, Larrondo L
Año: 2013.97:939-955.
Ref: Canessa P, Larrondo L. Environmental responses and the control of iron homeostasis in fungal systems. Applied Microbiology And Biotechnology. 2013;97(3):939-955.

22
The Paleozoic Origin Of Enzymatic Lignin Decomposition Reconstructed From 31 Fungal Genomes
Autor(es): Floudas D , Binder M , Riley R , Barry K, Blanchette R, Henrissat B , Martinez A, Otillar R, Spatafora J, Larrondo L, Et Al.
Año: 2012.336:1717-1719.
Ref: Floudas D , Binder M , Riley R , Barry K, Blanchette R, Henrissat B , Martinez A, Otillar R, Spatafora J, Larrondo L, et al.. The Paleozoic Origin of Enzymatic Lignin Decomposition Reconstructed from 31 Fungal Genomes. Science. 2012;336(6089):1717-1719.

23
High-Resolution Spatiotemporal Analysis Of Gene Expression In Real Time: In Vivo Analysis Of Circadian Rhythms In Neurospora Crassa Using A Frequency-Luciferase Translational Reporter
Autor(es): Larrondo L, Loros J, Dunlap J
Año: 2012.49:681-683.
Ref: Larrondo L, Loros J, Dunlap J. High-resolution spatiotemporal analysis of gene expression in real time: In vivo analysis of circadian rhythms in Neurospora crassa using a FREQUENCY-luciferase translational reporter. Fungal Genetics And Biology. 2012;49(9):681-683.

24
High-Resolution Spatiotemporal Analysis Of Gene Expression In Real Time: In Vivo Analysis Of Circadian Rhythms In Neurospora Crassa Using A Frequency-Luciferase Translational Reporter
Autor(es): Luis F. Larrondo, Jennifer J. Loros, Jay C. Dunlap
Año: 2012.49:681-683.doi: 10.1016/j.fgb.2012.06.001
Ref: Luis F. Larrondo, Jennifer J. Loros, Jay C. Dunlap. High-resolution spatiotemporal analysis of gene expression in real time: in vivo analysis of circadian rhythms in Neurospora crassa using a FREQUENCY-Luciferase translational reporter. Fungal Genetics And Biology. 2012;49(9):681-683.

25
Comparative Genomics Of Ceriporiopsis Subvermispora And Phanerochaete Chrysosporium Provide Insight Into Selective Ligninolysis
Autor(es): Fernandez E , Ruiz F, Ferreira P , Floudas D, Hibbett D, Canessa P, Larrondo Lf , James T, Seelenfreund D , Lobos S , Vicuna J, Et Al.
Año: 2012.109:5458-5463.
Ref: Fernandez E , Ruiz F, Ferreira P , Floudas D, Hibbett D, Canessa P, Larrondo LF , James T, Seelenfreund D , Lobos S , Vicuña J, et al.. Comparative genomics of Ceriporiopsis subvermispora and Phanerochaete chrysosporium provide insight into selective ligninolysis. Proceedings Of The National Academy Of Sciences Of The United States Of Ame. 2012;109(14):5458-5463.

26
Characterization Of Pir1, A Gata Family Transcription Factor Involved In Iron Responses In The White-Rot Fungus Phanerochaete Chrysosporium
Autor(es): Canessa P , Munoz F , Vicuna J, Larrondo L
Año: 2012.49:626-634.
Ref: Canessa P , Muñoz F , Vicuña J, Larrondo L. Characterization of PIR1, a GATA family transcription factor involved in iron responses in the white-rot fungus Phanerochaete chrysosporium. Fungal Genetics And Biology. 2012;49(8):626-634.

27
Molecular And Structural Modeling Of The Phanerochaete Flavido-Alba Extracellular Laccase Reveals Its Ferroxidase Structure
Autor(es): Rodriguez F, Suarez A, Lucas M, Larrondo Lf, De La Rubia T, Polaina J, Martinez J
Año: 2010.192:883-892.
Ref: Rodriguez F, Suarez A, Lucas M, Larrondo LF, De la Rubia T, Polaina J, Martinez J. Molecular and structural modeling of the Phanerochaete flavido-alba extracellular laccase reveals its ferroxidase structure. Archives Of Microbiology. 2010;192(11):883-892.

28
Genome, Transcriptome, And Secretome Analysis Of Wood Decay Fungus Postia Placenta Supports Unique Mechanisms Of Lignocellulose Conversion
Autor(es): Martinez D, Challacombe J, Morgenstern I, Hibbett D, Schmoll M, Kubicek C, Ferreira P, Ruiz-Duenas F, Martinez A, Kersten P, Hammel K, Wymelenberg A, Gaskell J, Lindquist E, Sabat G, Bondurant S, Larrondo L, Canessa P, Vicuna R, Yadav J, Doddapaneni H, Subramanian V, Pisabarro A, Lavin J, Oguiza J, Master E, Henrissat B, Coutinho P, Harris P, Magnuson J, Baker S, Bruno K, Kenealy W, Hoegger P, Kuees U, Ramaiya P, Lucash S, Salamov A, Shapiro H, Tu H, Chee C, Misra M, Xie G, Teter A, Yaver D, James T, Mokrejs M, Pospisek M, Grigoriev I, Brettin T, Rokhsar D, Berka R, Cullen D
Año: 2009.106:1954-1959.
Ref: Martínez D, Challacombe J, Morgenstern I, Hibbett D, Schmoll M, Kubicek C, Ferreira P, Ruíz-Dueñas F, Martínez A, Kersten P, Hammel K, Wymelenberg A, Gaskell J, Lindquist E, Sabat G, BonDurant S, Larrondo L, Canessa P, Vicuña R, Yadav J, Doddapaneni H, Subramanian V, Pisabarro A, Lavín J, Oguiza J, Master E, Henrissat B, Coutinho P, Harris P, Magnuson J, Baker S, Bruno K, Kenealy W, Hoegger P, Kuees U, Ramaiya P, Lucash S, Salamov A, Shapiro H, Tu H, Chee C, Misra M, Xie G, Teter A, Yaver D, James T, Mokrejs M, Pospisek M, Grigoriev I, Brettin T, Rokhsar D, Berka R, Cullen D. Genome, transcriptome, and secretome analysis of wood decay fungus Postia placenta supports unique mechanisms of lignocellulose conversion. Proceedings Of The National Academy Of Sciences Of The United States Of Ame. 2009;106(6):1954-1959.

29
An Eleven Amino Acid Residue Deletion Expands The Substrate Specificity Of Acetyl Xylan Esterase Ii (Axe Ii) From Penicillium Purpurogenum
Autor(es): Colombres M, Garate J, Lagos C, Araya-Secchi R, Norambuena P, Quiroz S, Larrondo L, Perez-Acle T, Eyzaguirre J
Año: 2008.22:19-28.
Ref: Colombres M, Gárate J, Lagos C, Araya-Secchi R, Norambuena P, Quiroz S, Larrondo L, Pérez-Acle T, Eyzaguirre J. An eleven amino acid residue deletion expands the substrate specificity of acetyl xylan esterase II (AXE II) from Penicillium purpurogenum. Journal Of Computer-Aided Molecular Design. 2008;22(1):19-28.

30
Functional Analysis Of The Endoxylanase B (Xynb) Promoter From Penicillium Purpurogenum
Autor(es): Diaz J, Chavez R, Larrondo L, Eyzaguirre J, Bull P
Año: 2008.54:133-141.
Ref: Díaz J, Chávez R, Larrondo L, Eyzaguirre J, Bull P. Functional analysis of the endoxylanase B (xynB) promoter from Penicillium purpurogenum. Current Genetics. 2008;54(3):133-141.

31
Genome Sequencing And Analysis Of The Biomass-Degrading Fungus Trichoderma Reesei (Syn. Hypocrea Jecorina)
Autor(es): Martinez D, Berka R, Henrissat B, Saloheimo M, Arvas M, Baker S, Chapman J, Chertkov O, Coutinho P, Cullen D, Danchin E, Grigoriev I, Harris P, Jackson M, Kubicek C, Han C, Ho I, Larrondo L, Lopez A, Magnuson J, Merino S, Misra M, Nelson B, Putnam N, Robbertse B, Salamov A, Schmoll M, Terry A, Thayer N, Westerholm-Parvinen A, Schoch C, Yao J, Barbote R, Nelson M, Detter C, Bruce D, Kuske C, Xie G, Richardson P, Rokhsar D, Lucas S, Rubin E, Dunn-Coleman N, Ward M
Año: 2008.26:553-560.
Ref: Martínez D, Berka R, Henrissat B, Saloheimo M, Arvas M, Baker S, Chapman J, Chertkov O, Coutinho P, Cullen D, Danchin E, Grigoriev I, Harris P, Jackson M, Kubicek C, Han C, Ho I, Larrondo L, López A, Magnuson J, Merino S, Misra M, Nelson B, Putnam N, Robbertse B, Salamov A, Schmoll M, Terry A, Thayer N, Westerholm-Parvinen A, Schoch C, Yao J, Barbote R, Nelson M, Detter C, Bruce D, Kuske C, Xie G, Richardson P, Rokhsar D, Lucas S, Rubin E, Dunn-Coleman N, Ward M. Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina). Nature Biotechnology. 2008;26(5):553-560.

32
Fully Codon-Optimized Luciferase Uncovers Novel Temperature Characteristics Of The Neurospora Clock
Autor(es): Gooch, V, Mehra A, Larrondo Lf, Fox J, Loros Jj, Dunlap Jc
Año: 2008.7:28-37.
Ref: Gooch, V, Mehra A, Larrondo LF, Fox J, Loros JJ, Dunlap JC. Fully codon-optimized luciferase uncovers novel temperature characteristics of the Neurospora clock. Eukaryotic Cell. 2008;7(1):28-37.

33
Cloning And Characterization Of The Genes Encoding The High-Affinity Iron-Uptake Protein Complex Fet3/ftr1 In The Basidiomycete Phanerochaete Chrysosporium
Autor(es): Larrondo L, Canessa P, Melo F, Polanco R, Vicuna R
Año: 2007.153:1772-1780.
Ref: Larrondo L, Canessa P, Melo F, Polanco R, Vicuna R. Cloning and characterization of the genes encoding the high-affinity iron-uptake protein complex Fet3/Ftr1 in the basidiomycete Phanerochaete chrysosporium. Microbiology-Sgm. 2007;153:1772-1780.

34
Structure And Transcriptional Impact Of Divergent Repetitive Elements Inserted Within Phanerochaete Chrysosporium Strain Rp-78 Genes
Autor(es): Larrondo L, Canessa P, Vicuna R, Stewart P, Vanden Wymelenberg A, Cullen D
Año: 2007.277:43-55.
Ref: Larrondo L, Canessa P, Vicuña R, Stewart P, Vanden Wymelenberg A, Cullen D. Structure and transcriptional impact of divergent repetitive elements inserted within Phanerochaete chrysosporium strain RP-78 genes. Molecular Genetics And Genomics. 2007;277(1):43-55.

35
The Band Mutation In Neurospora Crassa Is A Dominant Allele Of Ras-1 Implicating Ras Signaling In Circadian Output
Autor(es): Belden Wj, Larrondo Lf, Froehlich Ac, Shi M, Chen C, Loros Jj, Dunlap Jc
Año: 2007.21:1494-1505.
Ref: Belden WJ, Larrondo LF, Froehlich AC, Shi M, Chen C, Loros JJ, Dunlap JC. The band mutation in Neurospora crassa is a dominant allele of ras-1 implicating RAS signaling in circadian output. Genes & Development. 2007;21:1494-1505.

36
A Developmental Cycle Masks Output From The Circadian Oscillator Under Conditions Of Choline Deficiency In Neurospora
Autor(es): Shi M, Larrondo Lf, Loros Jj, Dunlap Jc
Año: 2007.104:20102-20107.
Ref: Shi M, Larrondo LF, Loros JJ, Dunlap JC. A developmental cycle masks output from the circadian oscillator under conditions of choline deficiency in Neurospora. Proceedings Of The National Academy Of Sciences Of The United States Of Ame. 2007;104(50):20102-20107.

37
Cloning And Functional Characterization Of The Gene Encoding The Transcription Factor Ace1 In The Basidiomycete Phanerochaete Chrysosporium
Autor(es): Polanco R, Canessa P, Rivas A, Larrondo L, Lobos S, Vicuna J
Año: 2006.39:71-82.
Ref: Polanco R, Canessa P, Rivas A, Larrondo L, Lobos S, Vicuña J. Cloning and Functional Characterization of the Gene Encoding the Transcription Factor Ace1 in the Basidiomycete Phanerochaete Chrysosporium. Biological Research. 2006;39:71-82.

38
Induction Of Cellular Prion Protein Gene Expression By Copper In Neurons
Autor(es): Varela-Nallar L, Toledo E, Larrondo L, Cabral A, Martins V, Inestrosa N
Año: 2006.290:271-281.
Ref: Varela-Nallar L, Toledo E, Larrondo L, Cabral A, Martins V, Inestrosa N. Induction of Cellular Prion Protein Gene Expression by Copper in Neurons. American Journal Of Physiology-Cell Physiology. 2006;290(1):271-281.

39
The Nop Gene From Phanerochaete Chrysosporium Encodes A Peroxidase With Novel Structural Features
Autor(es): Larrondo L, Gonzalez A, Perez-Acle T, Cullen D, Vicuna J
Año: 2005.116:167-173.
Ref: Larrondo L, González A, Pérez-Acle T, Cullen D, Vicuña J. The Nop Gene From Phanerochaete Chrysosporium Encodes a Peroxidase With Novel Structural Features. Biophysical Chemistry. 2005;116(2):167-173.

40
Incomplete Processing Of Peroxidase Transcripts In The Lignin Degrading Fungus Phanerochaete Chrysosporium
Autor(es): Stuardo M, Larrondo L, Vasquez M, Vicuna J, Gonzalez B
Año: 2005.242:37-44.
Ref: Stuardo M, Larrondo L, Vásquez M, Vicuña J, González B. Incomplete Processing of Peroxidase Transcripts in the Lignin Degrading Fungus Phanerochaete Chrysosporium. Fems Microbiology Letters. 2005;242(1):37-44.

41
Characterization Of A Multicopper Oxidase Gene Cluster In Phanerochaete Chrysosporium And Evidence Of Altered Splicing Of The Mco Transcripts
Autor(es): Larrondo L, Gonzalez B, Cullen D, Vicuna J
Año: 2004.150:2775-2783.
Ref: Larrondo L, González B, Cullen D, Vicuña J. Characterization of a Multicopper Oxidase Gene Cluster in Phanerochaete Chrysosporium and Evidence of Altered Splicing of the Mco Transcripts. Microbiology. 2004;150:2775-2783.

42
Genome Sequence Of The Lignocellulose Degrading Fungus Phanerochaete Chrysosporium Strain Rp78
Autor(es): Martinez D, Larrondo Lf, Putnam N, Gelpke Mds, Huang K, Chapman J, Helfenbein Kg, Ramaiya P, Detter Jc, Larimer F, Coutinho Pm, Henrissat B, Berka R, Cullen D, Rokhsar D
Año: 2004.22:695-700.
Ref: Martinez D, Larrondo LF, Putnam N, Gelpke MDS, Huang K, Chapman J, Helfenbein KG, Ramaiya P, Detter JC, Larimer F, Coutinho PM, Henrissat B, Berka R, Cullen D, Rokhsar D. Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78. Nature Biotechnology. 2004;22(6):695-700.

43
A Novel Extracellular Multicopper Oxidase From Phenerochaete Chrysosporium With Ferroxidase Activity
Autor(es): Larrondo L, Salas L, Melo F, Vicuna J, Cullen D
Año: 2003.69:6257-6263.
Ref: Larrondo L, Salas L, Melo F, Vicuña J, Cullen D. A Novel Extracellular Multicopper Oxidase From Phenerochaete Chrysosporium With Ferroxidase Activity. Applied And Environmental Microbiology. 2003;69(10):6257-6263.

44
Heterologous Expression Of Laccase Cdna From Ceriporiopsis Subvermispora Yields Copper-Activated Apoprotein And Complex Isoform Patterns
Autor(es): Larrondo L, Avila M, Salas L, Cullen D, Vicuna J
Año: 2003.149:1177-1182.
Ref: Larrondo L, Ávila M, Salas L, Cullen D, Vicuña J. Heterologous Expression of Laccase Cdna From Ceriporiopsis Subvermispora Yields Copper-Activated Apoprotein and Complex Isoform Patterns. Microbiology. 2003;149:1177-1182.

45
Isoenzyme Multiplicity And Characterization Of Recombinant Manganese Peroxidases From Ceriporiopsis Subvermispora And Phanerochaete Chrysosporium
Autor(es): Larrondo L, Lobos S, Stewart P, Cullen D, Vicuna J
Año: 2001.67:2070-2075.
Ref: Larrondo L, Lobos S, Stewart P, Cullen D, Vicuña J. Isoenzyme Multiplicity and Characterization of Recombinant Manganese Peroxidases From Ceriporiopsis Subvermispora and Phanerochaete Chrysosporium. Applied And Environmental Microbiology. 2001;67(5):2070-2075.

46
Enzymology And Molecular Genetics Of The Ligninolytic System Of The Basidiomycete Ceriporiopsis Sybvermispora
Autor(es): Lobos S, Tello M, Polanco R, Larrondo L, Manubens A, Salas L, Vicuna J
Año: 2001.81:992-997.
Ref: Lobos S, Tello M, Polanco R, Larrondo L, Manubens A, Salas L, Vicuña J. Enzymology and Molecular Genetics of the Ligninolytic System of the Basidiomycete Ceriporiopsis Sybvermispora. Current Science. 2001;81(8):992-997.

47
The Role Of Oxidative Stress In The Toxicity Induced By Amyloid B-Peptide In Alzheimer`s Disease
Autor(es): Miranda S, Opazo C, Larrondo L, Munoz F, Ruiz F, Leighton F, Inestrosa N
Año: 2000.62:633-648.
Ref: Miranda S, Opazo C, Larrondo L, Muñoz F, Ruíz F, Leighton F, Inestrosa N. The Role of Oxidative Stress in the Toxicity Induced by Amyloid B-Peptide in Alzheimer`S Disease. Progress In Neurobiology. 2000;62(6):633-648.

48
Characterization Of Three New Manganese Peroxidase Genes From The Ligninolytic Basidiomycete Ceriporiopsis Subvermispora
Autor(es): Tello M, Corsini G, Larrondo Lf, Salas L, Lobos S, Vicuna R
Año: 2000.1490:137-144.
Ref: Tello M, Corsini G, Larrondo LF, Salas L, Lobos S, Vicuna R. Characterization of three new manganese peroxidase genes from the ligninolytic basidiomycete Ceriporiopsis subvermispora. Biochimica Et Biophysica Acta-Gene Structure And Expression. 2000;1490(1-2):137-144.

49
Cloning And Molecular Analysis Of A Cdna And The Cs-Mnp1 Gene Encoding A Manganese Peroxidase Isoenzyme From The Lignin-Degrading Basidiomycete Cerioporiopsis Subvermispora
Autor(es): Lobos S, Larrondo L, Salas L, Karahanian E, Vicuna J
Año: 1998.206:185-193.
Ref: Lobos S, Larrondo L, Salas L, Karahanian E, Vicuña J. Cloning and Molecular Analysis of a CDNA and the Cs-Mnp1 Gene Encoding a Manganese Peroxidase Isoenzyme From the Lignin-Degrading Basidiomycete Cerioporiopsis Subvermispora. Gene. 1998;206(2):185-193.

50
Oxidation Reactions Catalyzed By Manganese Peroxidase Isoenzymes From Ceriporiopsis Subvermispora
Autor(es): Urzua U, Larrondo L, Lobos S, Larrain J, Vicuna J
Año: 1995.371:132-136.
Ref: Urzúa U, Larrondo L, Lobos S, Larraín J, Vicuña J. Oxidation Reactions Catalyzed by Manganese Peroxidase Isoenzymes From Ceriporiopsis Subvermispora. Febs Letters. 1995;371(2):132-136.



Libros y Capitulos de Libros


Circadian Rhythms: From Genes To Proteins And Back, In Less Than 24-Hours.
Autor(es): Larrondo, L. F.
Año: 2013:243-271.
Ref: Larrondo, L. F.. Circadian rhythms: from genes to proteins and back, in less than 24-hours.. In: Neurospora: genomics and molecular biology. Norfolk: Caister Academic Press; 2013. p. 243-271.

Phanerochaete Chrysosporium Genomics
Autor(es): Larrondo L, Vicuna J, Cullen D
Año: 2005:315-352.
Ref: Larrondo L, Vicuña J, Cullen D. Phanerochaete Chrysosporium Genomics. In: Applied Mycology and Biotechnology. Volume 5, Genes and Genomics. Elsevier; 2005. p. 315-352.