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Publicaciones Contacto

Microscopía Avanzada



Contacto

  • Nicole Salgado Cortes
    Coordinador Técnico
  • Anexo: 2714
  • Mail:
  • Fernanda Gárate
    Especialista microscopia Óptica
  • Anexo: 2715
  • Mail:
  • Alejandro Munizaga
    Especialista microscopia electrónica
  • Anexo: 2715
  • Mail:
  • Liseth Garibaldi S.
    Especialista microscopia electrónica
  • Anexo: 2715
  • Mail:
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La Unidad de Microscopía Avanzada tiene como misión poner al servicio de la comunidad científica sus instrumentos y la expertise de su equipo técnico para el avance del conocimiento en las diversas áreas de investigación, así como apoyar y fortalecer la formación de investigadores en programas de pregrado y postgrado de las distintas facultades de la Universidad. Además cuenta con un amplio rango de equipos de microscopía óptica y electrónica, y provee servicios de preparación de muestras y análisis de imágenes.

En esta sección es posible encontrar información de publicaciones relacionadas con nuestros servicios. En una primera sección se puede acceder a Publicaciones que han contado con el apoyo de nuestro equipamiento y personal. Una segunda seccion cuenta con publicaciones relacionadas con técnicas y métodos de preparación de muestras, además de otros documentos relacionados con investigaciones que se pueden realizar utilizando nuestros servicios.


Año Equipo Autores Título Revista Aplicación / Metodología Link
2018TECNAI+TIMELAPSEMarina Bartsakoulia, Angela Pyle, Diego Troncoso-Chandía, Josefa Vial-Brizzi, Marysol V Paz-Fiblas, Jennifer Duff, Helen…A novel mechanism causing imbalance of mitochondrial fusion and fission in human myopathies,Human Molecular Genetics, Volume 27, Issue 7, 1 April 2018, Pages 1186–1195.AplicaciónLinkTEM, microscopia, electronica, confocal, mutation, mitochondria, fibroblasts, dna mitochondrial,immunoblotting, skeletal muscles, myopathy, biopsy of muscle, respiratory chain
2012FV1000Riquelme E., Abarca J., Campusano JM. y Bustos G.…A NR2B-dependent decrease in the expression of trkB receptors precedes the disappearance of dopaminergic cells in substantia nigra in a rat model of presymptomatic Parkinson’s disease.Parkinson’s Disease. 2012;2012:129605.AplicaciónLinkmicroscopia, confocal, Parkinson disease, Dopaminergic, presymptomatic, immunohistochemical, tyrosine hydroxylase
2008FV1000Lee J, Retamal C., Cuitiño L, Caruano-Yzermans A., Shin JE, van Kekrkhof P, Marzolo MP and Bu G.…Adaptor protein sorting nexin 17 regulates amyloid precursor protein trafficking and processing in the early endosomes.Journal of Biological Chemistry Apr 25;283(17):11501-8AplicaciónLinkmicroscopia, confocal, colocalizacion, Immunofluorescence, Alzheimer disease, amyloid precursor protein, neurodegeneration, nexin, adaptor protein
2019ESPECTRALContreras O., Rossi F. M., Brandan E.…Adherent muscle connective tissue fibroblasts are phenotypically and biochemically equivalent to stromal fibro/adipogenic progenitors.Matrix Biology Plus Volume 2, May 2019, 100006AplicaciónLinkmicroscopia, confocal, immunofluorescence, Skeletal muscle, PDGFRα, FAPs, Fibrosis, Mesenchymal progenitors,TGF-β signaling
2020TIMELAPSEStuardo N, Moya-Alvarado G, Ramírez C, Schiavo G, Bronfman F (2020)…An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons. J Vis Exp.JoVE Journal NeuroscienceAplicaciónLinkmicroscopia, confocal, neuroscience, video Protocol, Cellular Trafficking, neurons,
2015SPINNING DISKPasten C, Cerda J, Jausoro I, Court FA, Cáceres A, Marzolo MP…ApoER2 and Reelin are expressed in regenerating peripheral nerve and regulate Schwann cell migration by activating the Rac1 GEF protein, Tiam1Molecular and Cellular Neuroscience 16;69:1-11AplicaciónLinkmicroscopia, confocal, FRET, ApoER2, Reelin, Proteolytic processing, Sciatic nerve, Schwann cells, Migration, Rho GTPases, PAR3, Tiam1
2018Bignante EA, et al.…APP/Go protein Gβγ-complex signaling mediates Aβ degeneration and cognitive impairment in Alzheimer’s disease modelsNeurobiology of Aging 64, 44-57AplicaciónLinkmicroscopia, confocal, neurosciencie, Alzheimer, Amyloid β (Aβ), Amyloid precursor protein (APP), Go protein, Gβγ complex, Degeneration, 3xTg-AD mice
2018SPINNING DISK/TIMELAPSEHernández, D.E., Salvadores, N.A., Moya-Alvarado, G., Catalán, R.J., Bronfman, F.C., Court, F.A.…Axonal degeneration induced by glutamate excitotoxicity is mediated by necroptosisJournal of Cell Science 2018AplicaciónLinkmicroscopia, confocal, time-lapse, calcium imaging, Neurodegeneration, Axonal degeneration, Excitotoxicity, Necroptosis
2018TIMELAPSEMoya-Alvarado, G., Gonzalez, A., Stuardo., N., Bronfman, F.C.…Brain-Derived Neurotrophic Factor (BDNF) Regulates Rab5-Positive Early Endosomes in Hippocampal Neurons to Induce Dendritic BranchingFrontiers in Cellular Neuroscience 12:493AplicaciónLinkmicroscopia, confocal, time-lapse, colocalization, Neurotrophins, BDNF, TrkB, Rab5, Rab11, endosomes, dendritic branching, neuron
2019TIMELAPSEGutierrez DA, Vargas LM, Chandía-Cristi A, Dela Fuente C, Alvarez AR…c-Abl defficient mice provides synaptic resiliency against Ab-oligomersFrontiers in Cellular Neuroscience 2019, 13:526AplicaciónLinkmicroscopia, confocal, immunofluorescence,Alzheimer’s disease, c-Abl tyrosine kinase, synapse, Aβ-oligomers, dendritic spines
2019TECNAI+TIMELAPSEEscudero, C.A., Cabeza, C., Moya-Alvarado, G., Maloney, M.T., Flores, C.M., Chengbiao, W., Court, F.C., Mobley, W., Bron…c-Jun N-terminal kinase is required for p75 neurotrophin receptor internalization toward apoptotic signaling endosomes in axons of sympathetic neuronsScientific Reports 2019AplicaciónLinkTEM, microscopia, electronica, confocal, immunofluorescence, endocytic sorting, neuronal death, JNK, p75 signaling
2019ESPECTRALOsvaldo Contreras, Meilyn Cruz-Soca, Marine Theret, Hesham Soliman, Lin Wei Tung, Elena Groppa, Fabio M. Rossi, Enrique …Cross-talk between TGF-β and PDGFRα signaling pathways regulates the fate of stromal fibro-adipogenic progenitors.Journal of Cell Science 132, 232157AplicaciónLinkmicroscopia, confocal, immunofluorescence, Regeneration, Fibrosis, Myofibroblast, Mesenchymal progenitors, Fibroblast, Skeletal muscle
2020FV1000Yarur HE, González MP, Verbel-Vergara D, Andrés ME, Gysling K.…Crosstalk between dopamine D1 and corticotropin releasing factor type 2 receptors leads to occlusion of their ERK1/2 signaling.Journal of Neurochemistry 2020 Mar 25AplicaciónLinkmicroscopia, confocal, dopamine D1 receptor, ERK signaling, heteromerization, synaptosomes, type‐2α CRF receptor
2019ESPECTRALContreras O., Vio C.P., Murphy-Ullrich J.E., Lipson K.E., Brandan E.…Denervation-induced skeletal muscle fibrosis is mediated by CTGF/CCN2 independently of TGF-β.Matrix Biology 2019 Sep;82:20-37AplicaciónLinkmicroscopia, confocal, colocalization Denervation, Fibrosis, CTGF/CCN2, Skeletal muscle, FG-3019
2020TIRFPaola Soto, Pablo S. Gaete, Christian Fuentes, Benjamin Lozano, Pamela A. Naulin, Xavier F. Figueroa and Nelson Patricio…Function of P2X4 Receptors Is Directly Modulated by a 1:1 Stoichiometric Interaction With 5-HT3A ReceptorsFrontiers in cellular NeuroscienceAplicaciónLinkmicroscopia, TIRF, AFM, receptor-receptor interaction, atomic force microscopy, stoichiometry, P2X4 receptor, 5-HT3A receptor, intracellular Ca2+, ATP
2020FV1000Yarur HE, Vega-Quiroga I, González MP, Noches V, Thomases DR, Andrés ME, Ciruela F, Tseng KY,…Inhibitory Control of Basolateral Amygdalar Transmission to the Prefrontal Cortex by Local Corticotrophin Type 2 ReceptorInternational Journal of Neuropsychopharmacology 2019 Dec 4AplicaciónLinkmicroscopia, confocal, Immunofluorescence, colocalization, Prefrontal Cortex, Corticotrophin receptor, amygdalar projections
2012FV1000Yévenes LF, Klein A, Castro JF, Marín T, Leal N, Leighton F, Alvarez AR, Zanlungo S…Lysosomal vitamin E accumulation in Niemann-Pick type C disease.Biochimica et Biophysica Acta 1822:150-160 (16)AplicaciónLinkmicroscopia, confocal, colocalization, Vitamin E, Niemann–Pick C, Cholesterol, Lysosomes
2015ESPECTRALSilva B, Molina-Fernández C, Ugalde MB, Tognarelli EI, Angel C and Campusano JM.…Muscarinic ACh receptors contribute to aversive olfactory learning in Drosophila.Neural Plasticity 2015;2015:658918AplicaciónLinkmicroscopia, confocal, Memory Consolidation, Olfactory Learning, Drosophila, muscarinic ACh receptors
2018TECNAIAndrea Del Campo, Ignacio Contreras-Hernández, Mauricio Castro-Sepúlveda, Cristian A Campos, Reinaldo Figueroa, María Fl…Muscle Function Decline and Mitochondria Changes in Middle Age Precede Sarcopenia in MiceAging, 10 (1), 34-55AplicaciónLinkTEM, microscopia, electronica, skeletal muscle fibers, sarcopenia, mitochondrial dynamics, SR-mitochondria coupling
2018SPINNING DISKJuan M Zolezzi 1 2, Carolina B Lindsay 1, Felipe G Serrano 1, Roxana C Ureta 1, Cristina Theoduloz 3, Guillermo Schmeda-…Neuroprotective Effects of Ferruginol, Jatrophone, and Junicedric Acid Against Amyloid-β Injury in Hippocampal NeuronsJournal of Alzheimer’s Disease, 63, 705-723AplicaciónLinkmicroscopia, confocal, cell imaging, Amyloid-β, diterpenes, ferruginol, jatrophone, junicedric acid, neuroprotection
2018TECNAIPedro Cisternas 1, Juan M Zolezzi 1, Carolina Lindsay 1, Daniela S Rivera 1 2, Alexis Martinez 1, Francisco Bozinovic 2 …New Insights into the Spontaneous Human Alzheimer’s Disease-Like Model Octodon degus: Unraveling Amyloid-β Peptide Aggregation and Age-Related Amyloid PathologyJournal of Alzheimer’s Disease 66, 1145-1163AplicaciónLinkmicroscopia, TEM, electronica, Alzheimer’s disease, Aβ peptide, natural model, Octodon degus, senile plaques
2020ESPECTRALSebastián Moreno, Javier Canales, Lilan Hong, Dana Robinson, Adrienne H.K. Roeder, Rodrigo A. Gutierrez…Nitrate Defines Shoot Size through Compensatory Roles for Endoreplication and Cell Division in Arabidopsis thalianaCurrent Biology VOLUME 30, ISSUE 11, P1988-2000.E3, JUNE 08, 2020AplicaciónLinkmicroscopia, confocal, cell expansion, endoreplication, ploidy, cell proliferation, organ size
2011TECNAILevtchenko E, De Leo MG Vicinanza M, Antonella Di Campli , Polishchuk E, Santoro M, Di Tullio G, Godi A, , Polishchuk R,…OCRL controls trafficking through early endosomes via PtdIns4,5P2-dependent regulation of endosomal actin.The EMBO Journal 30(24):4970-4985AplicaciónLinkTEM, microscopia, electronica, early endosomes, endosomal actin, trafficking, Lowe syndrome
2014SPINNING DISKLeyton V., Goles, N.I., Fuenzalida-Uribe, N. y Campusano JM.…Octopamine and Dopamine differentially modulate the nicotine-induced calcium response in Drosophila Mushroom Body Kenyon cells.Neuroscience Letters 560: 16-20, 2014.AplicaciónLinkmicroscopia, confocal, Dopamine, Octopamine, Nicotinic acetylcholine receptor, Drosophila, Calcium
2014TECNAIEscudero C, Lazo O, Galleguillos C, Parraguez J, Lopez-Verrilli M, Cabeza C, Leon L, Saeed U, Retamal C, Gonzalez A, Mar…p75 neurotrophin receptor evades the endolysosomal route, favouring multivesicular bodies specialised for exosomal release in neuronal cells.Journal of Cell Science. 2014 Feb 25AplicaciónLinkTEM, microscopia, electronica, MVBs,Traffic, Endocytosis, Neurotrophins, p75
2019TECNAIPedro Cisternas 1, Carolina A Oliva 1, Viviana I Torres 1, Daniela P Barrera 1, Nibaldo C Inestrosa…Presymptomatic Treatment With Andrographolide Improves Brain Metabolic Markers and Cognitive Behavior in a Model of Early-Onset Alzheimer’s DiseaseFrontiers in Neurocience 13AplicaciónLinkTEM, microscopia, electronica, andrographolide, Wnt signaling, glucose metabolism, Alzheimer’s disease, neuroprotection
2019TIRFIbañez-Vega, J., Del Valle Batalla, F., Saez, J. J., Soza, A., & Yuseff, M.…Proteasome Dependent Actin Remodeling Facilitates Antigen Extraction at the Immune Synapse of B Cells.Frontiers in Immunology, 10(B cell Biology), 1–13AplicaciónLinkmicroscopia, confocal, TIRF, B cells, immune synapse, cell polarity lysosomes proteasome, cytoskeleton remodeling, Arp2, MG-132, epoxomicin
2019TECNAICarreras-Sureda A, et al.…Publisher Correction: Non-canonical function of IRE1α determines mitochondria-associated endoplasmic reticulum composition to control calcium transfer and bioenergeticsNature Cell Biology 21, 913AplicaciónLinkTEM, microscopia, electronica, Mitochondria-associated membranes, bioenergetics, calcium transfer, endoplasmic reticulum
2020TECNAIMauricio Castro-Sepulveda, Sebastian Jannas-Vela, Rodrigo Fernández-Verdejo, Daniela Ávalos-Allele, German Tapia, Claudi…Relative lipid oxidation associates directly with mitochondrial fusion phenotype and mitochondria-sarcoplasmic reticulum interactions in human skeletal muscleAmerican Journal of PhysiologyAplicaciónLinkTEM, microscopia, electronica, fat oxidation, lipid droplet, mitochondria dynamic, organelles dynamic, respiratory quotient
2019TIRF-EPIValle-Tenney, Roger, et al.…Role of hypoxia in skeletal muscle fibrosis: Synergism between hypoxia and TGF-β signaling upregulates CCN2/CTGF expression specifically in muscle fibersMatrix Biology (2019).AplicaciónLinkmicroscopia, confocal, TIRF, hypoxia, keletal muscle, fibrosis, TGF-β signaling
2019TIMELAPSEConstanza Gonzalez, Pablo Guzman, Carlos Oliva, JC, et all.…Slit neuronal secretion coordinates optic lobe morphogenesis in DrosophilaDevelopmental BiologyAplicaciónLinkmicroscopia, confocal, Drosophila, morphogenesis, Slit protein, Medulla neurons, visual system
2020ESPECTRALContreras, O., Soliman, H., Theret, M., Rossi, F. M., & Brandan, E.…TGF-β-driven downregulation of the transcription factor TCF7L2 affects Wnt/β-catenin signaling in PDGFRα+ fibroblastsJournal of Cell ScienceAplicaciónLinkmicroscopia, confocal, Myofibroblasts, Stem cells, Regeneration, MSCs, PDGFRα, Myoblasts
2019TECNAIBerkowitz, L., Pardo-Roa, C., Ramírez, G., Vallejos, O. P., Sebastián, V. P., Riedel, C. A., … & Bueno, S. M.…The absence of interleukin 10 affects the morphology, differentiation, granule content and the production of cryptidin-4 in Paneth cells in mice.PloS one, 14(9), e0221618.AplicaciónLinkTEM, microscopia, electronica, interleukin, morphology, differentiation, Paneth cells, secretory granules
2019FV1000Hector E. Yarur, Maria Estela Andrés and Katia Gysling…Type 2β Corticotrophin Releasing Factor Receptor Forms a Heteromeric Complex With Dopamine D1 Receptor in Living CellsFrontiers in Pharmacology 2020 Jan 8;10:1501.AplicaciónLinkmicroscopia, confocal, Immunofluorescence, D1R (dopamine D1 receptor), heteromer, GPCR (G protein-coupled receptors, A7r5 cells, CRF2β
2019TECNAI+ESPECTRALRamos-Fernández E, et al.…Wnt-7a Stimulates Dendritic Spine Morphogenesis and PSD-95 Expression Through Canonical SignalingMolecular Neurobiology 56, 1870-1882AplicaciónLinkTEM, microscopia, electronica, confocal, transfection, Wnt signaling, Dendritic spine plasticity, PSD-95, TCF/LEF
2019TECNAICisternas P, et al.…Wnt-induced activation of glucose metabolism mediates the in vivo neuroprotective roles of Wnt signaling in Alzheimer diseaseJournal of Neurochemistry 149, 54-72AplicaciónLinkTEM, microscopia, electronica, confocal, Alzheimer disease, glucose metabolism, neuroprotection, Wnt signaling
2018TECNAIRíos JA, Godoy JA, Inestrosa NC…Wnt3a ligand facilitates autophagy in hippocampal neurons by modulating a novel GSK-3β-AMPK axisCell Communication and Signaling 16AplicaciónLinkTEM, microscopia, electronica, Neuron, Wnt signaling, AMPK, Autophagy, Metabolism
2019ESPECTRAL/FV1000Sebastian B. Arredondo Fernanda G. Guerrero Andrea Herrera‐Soto Joaquin Jensen‐Flores Daniel B. Bustamante Alejandro Oña…Wnt5a promotes differentiation and development of adult-born neurons in the hippocampus by noncanonical Wnt signalingStem Cells JournalAplicaciónLinkmicroscopia, confocal, neuroscience, wnt5a, differentiation, development, wnt signaling, hippocampus